Anticorps Monoclonal anti-ULK1

ULK1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7G1

N° de cat : 68445-1-PBS

Synonymes

1D7G1, ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, hATG1

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Galerie de données de validation

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  WB analysis of PC-3 using 68445-1-Ig (same clone as 68445-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68445-1-Ig (same clone as 68445-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68445-1-Ig (same clone as 68445-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68445-1-PBS cible ULK1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ULK1 Protéine recombinante Ag22397
• Nom complet: unc-51-like kinase 1 (C. elegans)
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: NM_003565
• Symbole du gène: ULK1
• Identification du gène (NCBI): 8408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

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