Anticorps Recombinant de lapin anti-UMP/CMP kinase

UMP/CMP kinase Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251233A1

N° de cat : 86475-3-RR

Synonymes

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

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Galerie de données de validation

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  WB analysis using 86475-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86475-3-RR (CMPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86475-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86475-3-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules U-251, tissu pancréatique de rat, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86475-3-RR cible UMP/CMP kinase dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UMP/CMP kinase Protéine recombinante Ag1920
• Nom complet: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Masse moléculaire calculée: 228 aa, 26 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC014961
• Symbole du gène: CMPK1
• Identification du gène (NCBI): 51727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

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