Anticorps Polyclonal de lapin anti-UNC119

UNC119 Polyclonal Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13065-1-AP

Synonymes

unc 119 homolog (C. elegans), RG4, Retinal protein 4, Protein unc-119 homolog A, Protein unc 119 homolog A

---

Galerie de données de validation

• 

  IF Staining of MDCK using 13065-1-AP

  Immunofluorescent analysis of MDCK cells using 13065-1-AP (UNC119 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

---

Applications testées

• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

13065-1-AP cible UNC119 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UNC119 Protéine recombinante Ag3757
• Nom complet: unc-119 homolog (C. elegans)
• Masse moléculaire calculée: 240 aa, 27 kDa
• Numéro d’acquisition GenBank: BC027176
• Symbole du gène: UNC119
• Identification du gène (NCBI): 9094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human UNC119 protein shares strong homology with the C. elegans unc119 protein which was first discovered in C. elegans on the basis of a spontaneous mutation affecting locomotion, feeding behavior and chemosensation. UNC119 is predominantly expressed in retina, in photoreceptor synapses and inner segments, with much lower expression in several other tissues. UNC119 is involved in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. It is required for G protein trafficking in sensory neurons.

Publications

Species	Application	Title
human	IHC	Int J Mol Sci; Ribbon Synapses and Retinal Disease: Review; Authors - Courtney E Frederick
Mouse	WB	Life Sci Alliance; UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia; Authors - Youhani Samarakoon

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Francesc R (Verified Customer) (06-14-2022)

Antibody works nicely by Western blot, specifically recognizing band of roughly the expected size. Attached image shows blot of CRISPR clones: four still contain UNC119 and all the others without the band are the UNC119-KO clones.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

UNC119 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:13065-1-AP)

{{ptg:RelatedPrimaryAntibodies}}