Anticorps Polyclonal de lapin anti-UNC13A

UNC13A Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 55053-1-AP

Synonymes

UNC13A/Munc13-1, Munc13-1, KIAA1032, Munc13 1, Protein unc 13 homolog A

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Galerie de données de validation

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  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55053-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55053-1-AP

  IP result of anti-UNC13A (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:10000) with mouse brain tissue lysate 1720 ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55053-1-AP cible UNC13A dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-13 homolog A (C. elegans)
• Masse moléculaire calculée: 193 kDa
• Poids moléculaire observé: 193-210 kDa
• Numéro d’acquisition GenBank: NM_001080421
• Symbole du gène: UNC13A
• Identification du gène (NCBI): 23025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Publications

Species	Application	Title
human	WB	Nature; TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.; Authors - X Rosa Ma
mouse	WB	Elife; Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.; Authors - Fushun Fan
	CoIP	Cell Rep; Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.; Authors - Hao Zhou
mouse,human	WB,IF	Sci Adv; Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release; Authors - Hong Zhang
Mouse		Antioxidants (Basel); Antioxidant Effects of DPP-4 Inhibitors in Early Stages of Experimental Diabetic Retinopathy; Authors - Hugo Ramos

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