Anticorps Polyclonal de lapin anti-UNC13A

UNC13A Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55053-1-PBS

Synonymes

UNC13A/Munc13-1, Munc13-1, KIAA1032, Munc13 1, Protein unc 13 homolog A

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Galerie de données de validation

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  WB analysis using 55053-1-AP (same clone as 55053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 55053-1-AP (same clone as 55053-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55053-1-AP (same clone as 55053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 55053-1-AP (same clone as 55053-1-PBS)

  IP result of anti-UNC13A (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:10000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55053-1-PBS cible UNC13A dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-13 homolog A (C. elegans)
• Masse moléculaire calculée: 193 kDa
• Poids moléculaire observé: 193-210 kDa
• Numéro d’acquisition GenBank: NM_001080421
• Symbole du gène: UNC13A
• Identification du gène (NCBI): 23025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

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