Anticorps Monoclonal anti-UNC13A

UNC13A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C3A9

N° de cat : 68483-1-PBS

Synonymes

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A

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Galerie de données de validation

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  WB analysis using 68483-1-Ig (same clone as 68483-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68483-1-Ig (UNC13A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68483-1-Ig (same clone as 68483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68483-1-Ig (same clone as 68483-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68483-1-Ig (same clone as 68483-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using UNC13A antibody (68483-1-Ig, Clone: 3C3A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68483-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68483-1-PBS cible UNC13A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-13 homolog A (C. elegans)
• Masse moléculaire calculée: 193 kDa
• Poids moléculaire observé: 193-210 kDa
• Numéro d’acquisition GenBank: NM_001080421
• Symbole du gène: UNC13A
• Identification du gène (NCBI): 23025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

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