Anticorps Recombinant de lapin anti-UNC13A

UNC13A Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240017A6

N° de cat : 83000-5-PBS

Synonymes

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

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Galerie de données de validation

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  WB analysis using 83000-5-RR (same clone as 83000-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83000-5-RR (UNC13A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  IP result of anti-UNC13A (IP:83000-5-RR, 4ug; Detection:83000-5-RR 1:1000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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Informations sur le produit

83000-5-PBS cible UNC13A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-13 homolog A (C. elegans)
• Masse moléculaire calculée: 193 kDa
• Poids moléculaire observé: 193-210 kDa
• Numéro d’acquisition GenBank: NM_001080421
• Symbole du gène: UNC13A
• Identification du gène (NCBI): 23025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

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