Anticorps Polyclonal de lapin anti-UNC13D/Munc13-4

UNC13D/Munc13-4 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16905-1-AP

Synonymes

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4

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Galerie de données de validation

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  WB analysis using 16905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16905-1-AP

  WB result of UNC13D/Munc13-4 antibody (16905-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC13D/Munc13-4 transfected K-562 cells.

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  WB analysis of human placenta using 16905-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16905-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16905-1-AP

  IP Result of anti-UNC13D/Munc13-4 (IP:16905-1-AP, 5ug; Detection:16905-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human thymus using 16905-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 16905-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de thymus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

16905-1-AP cible UNC13D/Munc13-4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UNC13D/Munc13-4 Protéine recombinante Ag10519
• Nom complet: unc-13 homolog D (C. elegans)
• Masse moléculaire calculée: 1090 aa, 103 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC067084
• Symbole du gène: UNC13D
• Identification du gène (NCBI): 201294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	Blood; Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.; Authors - Meeths Marie M
human	WB	Front Immunol; Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.; Authors - Donatella Galgano
human	WB,IF	Mol Biol Cell; A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.; Authors - Tarek Chehab