Anticorps Polyclonal de lapin anti-UNC45A

• Phare
• Validé par KD/KO

UNC45A Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 19564-1-AP

Synonymes

GC UNC45, GCUNC 45, GCUNC45, IRO039700, Protein unc 45 homolog A, SMAP 1, SMAP1, unc 45 homolog A (C. elegans), Unc 45A, UNC45A

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Galerie de données de validation

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  WB analysis of K-562 using 19564-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19564-1-AP

  WB result of UNC45A antibody (19564-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

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  WB analysis of mouse lung using 19564-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19564-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 19564-1-AP

  IP Result of anti-UNC45A (IP:19564-1-AP, 4ug; Detection:19564-1-AP 1:300) with K-562 cells lysate 3600ug.

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  IHC staining of human ovary tumor using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19564-1-AP (UNC45A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 19564-1-AP (UNC45A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human tonsil using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsil using 19564-1-AP (UNC45A antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, tissu ovarien de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu amygdalien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

19564-1-AP cible UNC45A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-45 homolog A (C. elegans)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_018671
• Symbole du gène: UNC45A
• Identification du gène (NCBI): 55898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.

Publications

Species	Application	Title
human	WB	FASEB J; A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.; Authors - Susana Lechuga ; KO Validated
rat	WB, IF	J Cell Sci; UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.; Authors - Juri Habicht
mouse	IHC,IF	Cells; UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.; Authors - Valentino Clemente

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (10-17-2019)

This Ab works well for western blotting (1:1000) but I did not get satisfactory results for Immunofluorescence (1:200) in human liver cell lines.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: H69, HUCCT-1

UNC45A Antibody Western Blot,Immunofluorescence, validation (1:1000 and 1:200 dilution) in H69, HUCCT-1 (Cat no:19564-1-AP)