Anticorps Polyclonal de lapin anti-UNC5A

UNC5A Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22068-1-AP

Synonymes

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

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Galerie de données de validation

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  WB analysis of HeLa using 22068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22068-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 22068-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22068-1-AP (UNC5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 22068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22068-1-AP (UNC5A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 22068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22068-1-AP (UNC5A Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of MCF-7 using 22068-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug UNC5A antibody (22068-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

22068-1-AP cible UNC5A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UNC5A Protéine recombinante Ag17155
• Nom complet: unc-5 homolog A (C. elegans)
• Masse moléculaire calculée: 842 aa, 93 kDa
• Poids moléculaire observé: 88-100 kDa
• Numéro d’acquisition GenBank: BC157824
• Symbole du gène: UNC5A
• Identification du gène (NCBI): 90249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.

Publications

Species	Application	Title
mouse	IF	Mol Psychiatry; YTHDF2 in dentate gyrus is the m6A reader mediating m6A modification in hippocampus-dependent learning and memory; Authors - Mengru Zhuang
human	IHC	Int J Clin Exp Pathol; The association of UNC5A expression with the clinicopathologic features and prognosis of radiotherapy in patients with non-small-cell lung cancer.; Authors - Silu Ding
human	WB	Cell Commun Signal; Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis; Authors - Lan Huang
human,mouse	IHC,IF	Cancers (Basel); Upregulation of Cell Surface Glycoproteins in Correlation with KSHV LANA in the Kaposi Sarcoma Tumor Microenvironment; Authors - Sara R Privatt