Anticorps Polyclonal de lapin anti-UNC5A-Specific

UNC5A-Specific Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 20239-1-AP

Synonymes

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

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Galerie de données de validation

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  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 20239-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug UNC5A-Specific antibody (20239-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20239-1-AP cible UNC5A-Specific dans les applications de WB, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-5 homolog A (C. elegans)
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 103 kDa
• Numéro d’acquisition GenBank: NM_133369
• Symbole du gène: UNC5A
• Identification du gène (NCBI): 90249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.