Anticorps Polyclonal de lapin anti-SUN1

SUN1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 24568-1-AP

Synonymes

KIAA0810, Protein unc 84 homolog A, Sad1/unc 84 protein like 1, SUN1, unc 84 homolog A (C. elegans), UNC84A

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Galerie de données de validation

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  WB analysis using 24568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24568-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24568-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 24568-1-AP

  IP result of anti-SUN1 (IP:24568-1-AP, 4ug; Detection:24568-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

24568-1-AP cible SUN1 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUN1 Protéine recombinante Ag21117
• Nom complet: unc-84 homolog A (C. elegans)
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC013613
• Symbole du gène: SUN1
• Identification du gène (NCBI): 23353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Nat Struct Mol Biol; DNA double-strand break-capturing nuclear envelope tubules drive DNA repair; Authors - Mitra Shokrollahi
human	WB	Acta Biochim Biophys Sin (Shanghai); SUN5, a testis-specific nuclear membrane protein, participates in recruitment and export of nuclear mRNA in spermatogenesis; Authors - Xiyi He ; KD Validated
mouse	WB	Int J Mol Sci; Electrical Stimulation Generates Induced Tumor-Suppressing Cells, Offering a Potential Option for Combatting Breast Cancer and Bone Metastasis; Authors - Changpeng Cui ; KD Validated
human	WB	Nat Commun; Nuclear membrane protein SUN2 promotes replication of flaviviruses through modulating cytoskeleton reorganization mediated by NS1; Authors - Yanxia Huang
human	WB	Adv Sci (Weinh); Attenuated Nuclear Tension Regulates Progerin-Induced Mechanosensitive Nuclear Wrinkling and Chromatin Remodeling; Authors - Ji-Eun Park

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