Anticorps Polyclonal de lapin anti-UPF1

• Phare
• Validé par KD/KO

UPF1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, RIP, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 23379-1-AP

Synonymes

ATP dependent helicase RENT1, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

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Galerie de données de validation

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  WB analysis using 23379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 23379-1-AP

  IP Result of anti-UPF1 (IP:23379-1-AP, 4ug; Detection:23379-1-AP 1:500) with Raji cells lysate 2000ug.

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  IF Staining of HepG2 using 23379-1-AP

  Immunofluorescent analysis of HepG2 cells using 23379-1-AP (UPF1 antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 23379-1-AP

  Immunofluorescent analysis of MCF-7 cells, using UPF1 antibody 23379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IF: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

23379-1-AP cible UPF1 dans les applications de WB, RIP, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UPF1 Protéine recombinante Ag16195
• Nom complet: UPF1 regulator of nonsense transcripts homolog (yeast)
• Masse moléculaire calculée: 123 kDa
• Poids moléculaire observé: 123-130 kDa
• Numéro d’acquisition GenBank: BC039817
• Symbole du gène: UPF1
• Identification du gène (NCBI): 5976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Publications

Species	Application	Title
human	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Breast Cancer Res; Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1.; Authors - Shuiyi Liu
human	WB	Bioengineered; The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.; Authors - Shizhen Sui
human	WB	J Exp Clin Cancer Res; PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.; Authors - Pingfu Hou
human	WB	Cell Death Dis; PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.; Authors - Rui Su
human	WB	Cell Rep; UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction.; Authors - Benjamin L Zaepfel ; KD Validated