Anticorps Recombinant de lapin anti-UPF1

UPF1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3O15

N° de cat : 82791-1-PBS

Synonymes

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

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Galerie de données de validation

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  WB analysis using 82791-1-RR (same clone as 82791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82791-1-RR (same clone as 82791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82791-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82791-1-RR against Human UPF1 were performed. The affinity constant is 50.7 nM.

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Informations sur le produit

82791-1-PBS cible UPF1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UPF1 Protéine recombinante Ag29057
• Nom complet: UPF1 regulator of nonsense transcripts homolog (yeast)
• Masse moléculaire calculée: 123 kDa
• Poids moléculaire observé: 123-130 kDa
• Numéro d’acquisition GenBank: BC039817
• Symbole du gène: UPF1
• Identification du gène (NCBI): 5976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

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