Anticorps Polyclonal de lapin anti-UPK2

UPK2 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 21149-1-AP

Synonymes

UP2, UPII, upk 2, UPK2, uroplakin 2, Uroplakin II

---

Galerie de données de validation

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 21149-1-AP (UPK2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 21149-1-AP (UPK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse bladder using 21149-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 21149-1-AP (UPK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en IHC: tissu vésical humain, tissu vésical de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

21149-1-AP cible UPK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UPK2 Protéine recombinante Ag15694
• Nom complet: uroplakin 2
• Masse moléculaire calculée: 184 aa, 19 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC113900
• Symbole du gène: UPK2
• Identification du gène (NCBI): 7379
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-2 (UPK2) is a single-pass type I membrane protein. UPK2 may play an important role in regulating the assembly of the AUM.

Publications

Species	Application	Title
human	IF	Biofabrication; Construction of a three-dimensional urothelium on-chip with barrier function based on urinary flow microenvironment; Authors - Changhao Hou
human	IF	Sci Rep; Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder.; Authors - Yuta Inoue
rat	WB,IHC	Int J Mol Sci; Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2-Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat; Authors - Hidenori Akaihata
human	IF	Adv Sci (Weinh); In Situ Transformable Nanoparticle Effectively Suppresses Bladder Cancer by Damaging Mitochondria and Blocking Mitochondrial Autophagy Flux; Authors - Yulin Lv
mouse	IF	Exp Cell Res; Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder; Authors - Md Kaykobad Hossain
mouse	WB	Biomark Res; S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome; Authors - Jiang Zhao

{{ptg:RelatedPrimaryAntibodies}}