Anticorps Polyclonal de lapin anti-UPK3A

UPK3A Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55229-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 55229-1-AP (same clone as 55229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55229-1-AP (UPK3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55229-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse bladder using 55229-1-AP (same clone as 55229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 55229-1-AP (Uroplakin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55229-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55229-1-PBS cible UPK3A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: uroplakin 3A
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31-33 kDa
• Numéro d’acquisition GenBank: NM_006953
• Symbole du gène: UPK3A
• Identification du gène (NCBI): 7380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.

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