Anticorps Polyclonal de lapin anti-UQCRB

• Phare
• Validé par KD/KO

UQCRB Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10756-1-AP

Synonymes

Complex III subunit 7, Complex III subunit VII, QCR7, QP C, QPC, UQBC, UQBP, UQCRB, UQPC

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Galerie de données de validation

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  WB analysis using 10756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10756-1-AP (UQCRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10756-1-AP

  IP Result of anti-UQCRB (IP:10756-1-AP, 3ug; Detection:10756-1-AP 1:600) with mouse heart tissue lysate 3200ug.

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  IHC staining of human breast cancer using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10756-1-AP (UQCRB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10756-1-AP (UQCRB Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below

Informations sur le produit

10756-1-AP cible UQCRB dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UQCRB Protéine recombinante Ag1127
• Nom complet: ubiquinol-cytochrome c reductase binding protein
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC005230
• Symbole du gène: UQCRB
• Identification du gène (NCBI): 7381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UQCRB(ubiquinol-cytochrome c reductase binding protein) is also named as UQBP, complex III subunit VII and belongs to the UQCRB/QCR7 family. It is a 13.4 kDa subunit of mitochondrial complex III, plays a crucial role in hypoxia-induced angiogenesis via mitochondrial reactive oxygen species (ROS)-mediated signaling(PMID:21215626). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Mol Cell; TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.; Authors - Emanuela Bottani
human	WB	Cell Metab; Mitochondrial complex III ROS regulate adipocyte differentiation.; Authors - Tormos Kathryn V KV
		Sci Adv; Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance.; Authors - Wanshu Ma ; KO Validated
human	WB	Nat Chem Biol; Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.; Authors - Christopher F Bennett
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang
mouse	WB	Neurobiol Aging; Up-regulation of key microRNAs, and inverse down-regulation of their predicted oxidative phosphorylation target genes, during aging in mouse brain.; Authors - Li Na N