Anticorps Monoclonal anti-UQCRC2

UQCRC2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

1A5G11

N° de cat : 67547-1-Ig

Synonymes

1A5G11, Complex III subunit 2, Core protein II, Cytochrome b-c1 complex subunit 2, mitochondrial, QCR2

---

Galerie de données de validation

• 

  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, rein de porc, tissu cardiaque de porc, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

67547-1-Ig cible UQCRC2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UQCRC2 Protéine recombinante Ag6874
• Nom complet: ubiquinol-cytochrome c reductase core protein II
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000484
• Symbole du gène: UQCRC2
• Identification du gène (NCBI): 7385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Circ Res; Endothelial FUNDC1 Deficiency Drives Pulmonary Hypertension; Authors - Yandong Pei
human	WB	J Transl Med; DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway; Authors - Lei Shi
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
mouse	WB	Eur J Pharmacol; Dimethyl Fumarate Improves Non-Alcoholic Fatty Liver Disease by Regulating SIRT1 Signal to Inhibit MAMs Over-Enrichment; Authors - Rui Zhang
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li

{{ptg:RelatedPrimaryAntibodies}}