Anticorps Polyclonal de lapin anti-UQCRQ

• Phare
• Validé par KD/KO

UQCRQ Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14975-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14975-1-AP (same clone as 14975-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14975-1-AP (same clone as 14975-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  IP result of anti-UQCRQ (IP:14975-1-AP, 4ug; Detection:14975-1-AP 1:1000) with HepG2 cells lysate 3360 ug. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (14975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14975-1-PBS cible UQCRQ dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UQCRQ Protéine recombinante Ag6886
• Nom complet: ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC001390
• Symbole du gène: UQCRQ
• Identification du gène (NCBI): 27089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

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