Anticorps Polyclonal de lapin anti-USF1

• Phare
• Validé par KD/KO

USF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 22327-1-AP

Synonymes

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

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Galerie de données de validation

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  WB analysis using 22327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 22327-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22327-1-AP (USF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 22327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22327-1-AP (USF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22327-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22327-1-AP (USF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

22327-1-AP cible USF1 dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USF1 Protéine recombinante Ag17789
• Nom complet: upstream transcription factor 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC035505
• Symbole du gène: USF1
• Identification du gène (NCBI): 7391
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

Publications

Species	Application	Title
human	WB	Genome Biol; Acute depletion of CTCF rewires genome-wide chromatin accessibility.; Authors - Beisi Xu ; KO Validated
human	WB	Elife; Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.; Authors - Hao Zhang ; KO Validated
human	WB,IHC	IUBMB Life; Upstream transcription factor 1 suppresses laryngeal squamous cell carcinoma progression through transcriptional activation of junctional adhesion molecule 3; Authors - Yue Jia
human,mouse	WB	J Oral Pathol Med; LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.; Authors - Xiuhe Zou ; KD Validated
human	WB,ChIP	Cell Signal; PUS1 facilitates cell migration in clear cell renal cell carcinoma through the promotion of mRNA pseudouridylation and the stabilization of the SMOX gene; Authors - Yao Chen ; KD Validated

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