Anticorps Monoclonal anti-USF1

USF1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8F1

N° de cat : 67114-1-PBS

Synonymes

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

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Galerie de données de validation

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  WB analysis using 67114-1-Ig (same clone as 67114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67114-1-Ig (same clone as 67114-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67114-1-Ig (same clone as 67114-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67114-1-Ig (same clone as 67114-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67114-1-Ig (same clone as 67114-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67114-1-Ig (same clone as 67114-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67114-1-Ig (same clone as 67114-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67114-1-PBS cible USF1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USF1 Protéine recombinante Ag17785
• Nom complet: upstream transcription factor 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 38-43 kDa
• Numéro d’acquisition GenBank: BC035505
• Symbole du gène: USF1
• Identification du gène (NCBI): 7391
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

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