Anticorps Polyclonal de lapin anti-USP1

• Phare
• Validé par KD/KO

USP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 14346-1-AP

Synonymes

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

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Galerie de données de validation

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  WB analysis of HEK-293 using 14346-1-AP

  WB result of USP1 antibody (14346-1-AP, 1:1000) with si-Control and si-USP1 transfected HEK293 cells.

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  WB analysis using 14346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14346-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14346-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14346-1-AP

  IP Result of anti-USP1 (IP:14346-1-AP, 3ug; Detection:14346-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 24 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below

Informations sur le produit

14346-1-AP cible USP1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP1 Protéine recombinante Ag5686
• Nom complet: ubiquitin specific peptidase 1
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 110 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC050525
• Symbole du gène: USP1
• Identification du gène (NCBI): 7398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).

Publications

Species	Application	Title
human	WB	Nat Chem Biol; A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.; Authors - Qin Liang
human	WB	Mol Cell; Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.; Authors - Ciccia Alberto A
human	WB,IF	Nat Commun; The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.; Authors - Kyungsoo Ha
human	WB	Nat Commun; hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma.; Authors - Pauline Herviou
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB,IHC,IF,IP	Theranostics; CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells; Authors - Apoorvi Tyagi