Anticorps Monoclonal anti-USP1

• Phare
• Validé par KD/KO

USP1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C10H6

N° de cat : 66069-1-PBS

Synonymes

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

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Galerie de données de validation

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  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66069-1-Ig (same clone as 66069-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66069-1-PBS cible USP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP1 Protéine recombinante Ag5904
• Nom complet: ubiquitin specific peptidase 1
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC050525
• Symbole du gène: USP1
• Identification du gène (NCBI): 7398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).

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