Anticorps Polyclonal de lapin anti-USP11

• Phare
• Validé par KD/KO

USP11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10244-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 10244-1-AP (same clone as 10244-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 10244-1-AP (same clone as 10244-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10244-1-AP (same clone as 10244-1-PBS)

  IP result of anti-USP11 (IP:10244-1-AP, 4ug; Detection:10244-1-AP 1:300) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10244-1-AP (same clone as 10244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10244-1-AP (USP11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10244-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10244-1-PBS cible USP11 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP11 Protéine recombinante Ag0296
• Nom complet: ubiquitin specific peptidase 11
• Masse moléculaire calculée: 963 aa, 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC000350
• Symbole du gène: USP11
• Identification du gène (NCBI): 8237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

{{ptg:RelatedPrimaryAntibodies}}