Anticorps Monoclonal anti-USP11

• Phare
• Validé par KD/KO

USP11 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G6E7

N° de cat : 67805-1-PBS

Synonymes

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

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Galerie de données de validation

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  WB analysis using 67805-1-Ig (same clone as 67805-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67805-1-Ig (same clone as 67805-1-PBS)

  WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67805-1-PBS cible USP11 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP11 Protéine recombinante Ag19058
• Nom complet: ubiquitin specific peptidase 11
• Masse moléculaire calculée: 963 aa, 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC000350
• Symbole du gène: USP11
• Identification du gène (NCBI): 8237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

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