Anticorps Monoclonal anti-USP13

• Phare
• Validé par KD/KO

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

6E8C10

N° de cat : 66176-1-Ig

Synonymes

6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3

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Galerie de données de validation

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  WB analysis using 66176-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HeLa using 66176-1-Ig

  WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells.

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  WB analysis of HepG2 using 66176-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 66176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 66176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 66176-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  IF Staining of HepG2 using 66176-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A375, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

66176-1-Ig cible USP13 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP13 Protéine recombinante Ag10506
• Nom complet: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Masse moléculaire calculée: 863 aa, 97 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC016146
• Symbole du gène: USP13
• Identification du gène (NCBI): 8975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
	WB	Front Mol Biosci; Deubiquitination of MITF-M Regulates Melanocytes Proliferation and Apoptosis.; Authors - Shuaishuai Hu ; KD Validated
mouse,human	WB	Biochim Biophys Acta Mol Basis Dis; USP13 inhibition exacerbates mitochondrial dysfunction and acute kidney injury by acting on MCL-1; Authors - Qian Wang

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