Anticorps Monoclonal anti-USP13

• Phare
• Validé par KD/KO

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6E8C10

N° de cat : 66176-1-PBS

Synonymes

6E8C10, EC:3.4.19.12, Isopeptidase T-3, ISOT3, ISOT-3

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Galerie de données de validation

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  WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

  WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66176-1-PBS cible USP13 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP13 Protéine recombinante Ag10506
• Nom complet: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Masse moléculaire calculée: 863 aa, 97 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC016146
• Symbole du gène: USP13
• Identification du gène (NCBI): 8975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

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