Anticorps Polyclonal de lapin anti-USP14

• Phare
• Validé par KD/KO

USP14 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 14517-1-AP

Synonymes

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

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Galerie de données de validation

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14517-1-AP

  WB result of USP14 antibody (14517-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

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  WB analysis of HeLa using 14517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14517-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14517-1-AP

  IP Result of anti-USP14 (IP:14517-1-AP, 4ug; Detection:14517-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IF Staining of HepG2 using 14517-1-AP

  Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 14517-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug USP14 antibody (14517-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Neuro-2a, cellules SKOV-3, tissu cardiaque de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:18000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

14517-1-AP cible USP14 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP14 Protéine recombinante Ag5979
• Nom complet: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52-60 kDa
• Numéro d’acquisition GenBank: BC003556
• Symbole du gène: USP14
• Identification du gène (NCBI): 9097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).

Publications

Species	Application	Title
human	WB,IF	Autophagy; USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination.; Authors - Arishya Sharma
pig	WB,IF,IP	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming ; KD Validated
human	IF	Cell Prolif; SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.; Authors - Jun Xu
human	WB	Front Pharmacol; SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis.; Authors - Tingting Zhu
human	IHC	J Biol Chem; The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities.; Authors - Guibin Fang
human	WB,IHC	Cancer Med; PMID: 30296012; Authors - Ying Fu ; KD Validated