Anticorps Monoclonal anti-USP14

USP14 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B12G3

N° de cat : 67746-1-PBS

Synonymes

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

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Galerie de données de validation

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  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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  WB analysis using 67746-1-Ig (same clone as 67746-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67746-1-Ig (same clone as 67746-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP14 antibody (67746-1-Ig, Clone: 3B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67746-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67746-1-PBS cible USP14 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP14 Protéine recombinante Ag6312
• Nom complet: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC003556
• Symbole du gène: USP14
• Identification du gène (NCBI): 9097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

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