Anticorps Recombinant de lapin anti-USP14

• Phare
• Validé par KD/KO

USP14 Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G12

N° de cat : 82128-1-PBS

Synonymes

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

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Galerie de données de validation

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  WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

  WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

  IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82128-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82128-1-PBS cible USP14 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: USP14 Protéine recombinante Ag5979
• Nom complet: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC003556
• Symbole du gène: USP14
• Identification du gène (NCBI): 9097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

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