Anticorps Monoclonal anti-USP15

• Phare
• Validé par KD/KO

USP15 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1C9G3

Avis (1)

N° de cat : 67557-1-Ig

Synonymes

1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15

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Galerie de données de validation

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  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67557-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HeLa using 67557-1-Ig

  WB result of USP15 antibody (67557-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells.

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  WB analysis of 4T1 using 67557-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67557-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67557-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67557-1-Ig cible USP15 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP15 Protéine recombinante Ag5712
• Nom complet: ubiquitin specific peptidase 15
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 112 kDa
• Numéro d’acquisition GenBank: BC020688
• Symbole du gène: USP15
• Identification du gène (NCBI): 9958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Cell Death Dis; The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression.; Authors - Xiaohong Xia ; KD Validated
human	WB	Endocr Relat Cancer; LncRNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer; Authors - Xiaoli Liu
human	WB,CoIP	J Cancer; USP15 promotes the progression of papillary thyroid cancer by regulating HMGB1 stability through its deubiquitination; Authors - Si-Si Wang ; KD Validated
mouse,human	WB,IP,IHC,IF	Nat Commun; Proteostatic reactivation of the developmental transcription factor TBX3 drives BRAF/MAPK-mediated tumorigenesis; Authors - Zhenlei Zhang
human	WB	ACS Pharmacol Transl Sci; Cell-Based Covalent-Capture Deubiquitinase Assay for Inhibitor Discovery; Authors - Megan N Doleschal
mouse,human	WB,IHC,IF	Mol Med; USP15 regulates radiation-induced DNA damage and intestinal injury through K48-linked deubiquitination and stabilisation of ATM; Authors - Ruiqiu Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shunbin (Verified Customer) (06-07-2023)

Great antibody for Western Blot. Very specific single band

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000

USP15 Antibody Western Blot validation (1:5,000 dilution) in (Cat no:67557-1-Ig)

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