Anticorps Monoclonal anti-USP15

• Phare
• Validé par KD/KO

USP15 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C9G3

N° de cat : 67557-1-PBS

Synonymes

1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15

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Galerie de données de validation

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  WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67557-1-Ig (same clone as 67557-1-PBS)

  WB result of USP15 antibody (67557-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67557-1-Ig (same clone as 67557-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP15 antibody (67557-1-Ig, Clone: 1C9G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67557-1-PBS cible USP15 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP15 Protéine recombinante Ag5712
• Nom complet: ubiquitin specific peptidase 15
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 112 kDa
• Numéro d’acquisition GenBank: BC020688
• Symbole du gène: USP15
• Identification du gène (NCBI): 9958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

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