Anticorps Polyclonal de lapin anti-USP18

USP18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12153-1-PBS

Synonymes

43 kDa ISG15-specific protease, EC:3.4.19.12, hUBP43, ISG15-specific-processing protease, ISG43

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Galerie de données de validation

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  WB analysis of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

  WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12153-1-AP (same clone as 12153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12153-1-AP (USP18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP18 antibody (12153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12153-1-PBS cible USP18 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP18 Protéine recombinante Ag2785
• Nom complet: ubiquitin specific peptidase 18
• Masse moléculaire calculée: 372 aa, 43 kDa
• Poids moléculaire observé: 43 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC014896
• Symbole du gène: USP18
• Identification du gène (NCBI): 11274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).

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