• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-USP19

USP19 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 25768-1-AP

Synonymes

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19



Applications testées

Résultats positifs en WBcellules HeLa, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231
Résultats positifs en IPcellules Jurkat

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

25768-1-AP cible USP19 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène USP19 Protéine recombinante Ag22646
Nom complet ubiquitin specific peptidase 19
Masse moléculaire calculée 1318 aa, 146 kDa
Poids moléculaire observé 70 kDa, 120 kDa, 150-180 kDa
Numéro d’acquisition GenBankBC146752
Symbole du gène USP19
Identification du gène (NCBI) 10869
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

Protocole

Product Specific Protocols
WB protocol for USP19 antibody 25768-1-APDownload protocol
IP protocol for USP19 antibody 25768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
IHC

Clin Transl Med

Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer

Authors - Xiaoqiang Zhang
humanWB,IP,IHC

Mol Ther

Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin

Authors - Apoorvi Tyagi
  • KD Validated
humanWB

J Cell Biol

USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites.

Authors - Peiyuan Chai
humanWB

Theranostics

USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2

Authors - Arun Pandian Chandrasekaran
  • KD Validated
human

Cell Mol Life Sci

Enhanced secretion of the amyotrophic lateral sclerosis ALS-associated misfolded TDP-43 mediated by the ER-ubiquitin specific peptidase USP19

Authors - Flavien Picard
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