Anticorps Polyclonal de lapin anti-USP19

• Phare
• Validé par KD/KO

USP19 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 25768-1-AP

Synonymes

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19

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Galerie de données de validation

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  WB analysis of Jurkat using 25768-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25768-1-AP

  WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25768-1-AP

  IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Informations sur le produit

25768-1-AP cible USP19 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP19 Protéine recombinante Ag22646
• Nom complet: ubiquitin specific peptidase 19
• Masse moléculaire calculée: 1318 aa, 146 kDa
• Poids moléculaire observé: 70 kDa, 120 kDa, 150-180 kDa
• Numéro d’acquisition GenBank: BC146752
• Symbole du gène: USP19
• Identification du gène (NCBI): 10869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
	IHC	Clin Transl Med; Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer; Authors - Xiaoqiang Zhang
human	WB,IP,IHC	Mol Ther; Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin; Authors - Apoorvi Tyagi ; KD Validated
human	WB	J Cell Biol; USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites.; Authors - Peiyuan Chai
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran ; KD Validated
human		Cell Mol Life Sci; Enhanced secretion of the amyotrophic lateral sclerosis ALS-associated misfolded TDP-43 mediated by the ER-ubiquitin specific peptidase USP19; Authors - Flavien Picard

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