Anticorps Polyclonal de lapin anti-USP19

• Phare
• Validé par KD/KO

USP19 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25768-1-PBS

Synonymes

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19

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Galerie de données de validation

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  WB analysis of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

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  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25768-1-AP (same clone as 25768-1-PBS)

  WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

  IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25768-1-PBS cible USP19 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP19 Protéine recombinante Ag22646
• Nom complet: ubiquitin specific peptidase 19
• Masse moléculaire calculée: 1318 aa, 146 kDa
• Poids moléculaire observé: 70 kDa, 120 kDa, 150-180 kDa
• Numéro d’acquisition GenBank: BC146752
• Symbole du gène: USP19
• Identification du gène (NCBI): 10869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

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