Anticorps Polyclonal de lapin anti-USP2

• Phare
• Validé par KD/KO

USP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 10392-1-AP

Synonymes

Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9

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Galerie de données de validation

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  WB analysis using 10392-1-AP

  NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10392-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10392-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10392-1-AP (USP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10392-1-AP (USP2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10392-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10392-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse embryo using 10392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules MCF-7, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu embryonnaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10392-1-AP cible USP2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP2 Protéine recombinante Ag0591
• Nom complet: ubiquitin specific peptidase 2
• Masse moléculaire calculée: 41 kDa, 69 kDa
• Poids moléculaire observé: 68 kDa, 41-45 kDa
• Numéro d’acquisition GenBank: BC002955
• Symbole du gène: USP2
• Identification du gène (NCBI): 9099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing. This antibody is specific to the isoform 1(USP2a) of USP2.

Publications

Species	Application	Title
human	WB	FASEB J; Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation.; Authors - Kitamura Hiroshi H
rat	WB	J Cell Mol Med; Ubiquitin-specific protease 2 regulates Ang Ⅱ-induced cardiac fibroblasts activation by up-regulating cyclin D1 and stabilizing β-catenin in vitro.; Authors - Qiong Xu ; KD Validated
mouse	WB	Mol Nutr Food Res; 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity.; Authors - Hee Yang
mouse	WB,IHC	Mediators Inflamm; Overexpression of Ubiquitin-Specific Protease 2 (USP2) in the Heart Suppressed Pressure Overload-Induced Cardiac Remodeling.; Authors - Junhui Xing
human,mouse	WB,IF,CoIP	J Cancer Res Clin Oncol; USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail; Authors - Chao Wei
human	IHC	Tumour Biol; USP2 promotes cell migration and invasion in triple negative breast cancer cell lines.; Authors - Qing Qu