Anticorps Polyclonal de lapin anti-USP24

• Phare
• Validé par KD/KO

USP24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 13126-1-AP

Synonymes

Deubiquitinating enzyme 24, EC:3.4.19.12, KIAA1057, Ubiquitin carboxyl-terminal hydrolase 24, Ubiquitin thioesterase 24

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Galerie de données de validation

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  WB analysis of HepG2 using 13126-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13126-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 13126-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13126-1-AP

  IP result of anti-USP24 (IP:13126-1-AP, 4ug; Detection:13126-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 13126-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP24 antibody (13126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

13126-1-AP cible USP24 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP24 Protéine recombinante Ag3779
• Nom complet: ubiquitin specific peptidase 24
• Masse moléculaire calculée: 294 kDa
• Poids moléculaire observé: 280-294 kDa
• Numéro d’acquisition GenBank: BC029660
• Symbole du gène: USP24
• Identification du gène (NCBI): 23358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer; Authors - Zeguo Sun
human	WB,IHC,IF	Nat Commun; USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.; Authors - Yi-Chang Wang ; KD Validated
mouse	WB	Nat Commun; A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.; Authors - Jung-Min Kim ; KD Validated
mouse		Nat Commun; ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.; Authors - Francesca Bufalieri
human	WB,IP	Cell Death Differ; USP24 promotes drug resistance during cancer therapy.; Authors - Shao-An Wang
human	WB	EMBO Mol Med; USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.; Authors - Katharina Engel

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