Anticorps Monoclonal anti-USP25

USP25 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B6A2

N° de cat : 67769-1-PBS

Synonymes

Ubiquitin thiolesterase 25, Ubiquitin thioesterase 25, Ubiquitin carboxyl-terminal hydrolase 25, EC:3.4.19.12, Deubiquitinating enzyme 25

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Galerie de données de validation

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  WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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  WB analysis using 67769-1-Ig (same clone as 67769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67769-1-Ig (same clone as 67769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67769-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67769-1-PBS cible USP25 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP25 Protéine recombinante Ag28067
• Nom complet: ubiquitin specific peptidase 25
• Masse moléculaire calculée: 125 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC015930
• Symbole du gène: USP25
• Identification du gène (NCBI): 29761
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

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