Anticorps Monoclonal anti-USP28

USP28 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E3D1

N° de cat : 67814-1-PBS

Synonymes

1E3D1, Deubiquitinating enzyme 28, EC:3.4.19.12, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28

---

Galerie de données de validation

• 

  WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67814-1-Ig (same clone as 67814-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP28 antibody (67814-1-Ig, Clone: 1E3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67814-1-PBS cible USP28 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP28 Protéine recombinante Ag11805
• Nom complet: ubiquitin specific peptidase 28
• Masse moléculaire calculée: 583aa,66 kDa; 1077aa,120 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC065928
• Symbole du gène: USP28
• Identification du gène (NCBI): 57646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP28 (Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

{{ptg:RelatedPrimaryAntibodies}}