Anticorps Polyclonal de lapin anti-USP3

• Phare
• Validé par KD/KO

USP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 12490-1-AP

Synonymes

EC:3.4.19.12, SIH003, Ubiquitin carboxyl-terminal hydrolase 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3

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Galerie de données de validation

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  WB analysis using 12490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12490-1-AP

  WB result of USP3 antibody (12490-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP3 transfected K-562 cells.

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  WB analysis of K-562 using 12490-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 12490-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12490-1-AP

  IP result of anti-USP3 (IP:12490-1-AP, 4ug; Detection:12490-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 12490-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12490-1-AP (USP3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HeLa using 12490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 12490-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12490-1-AP (USP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules Jurkat, cellules K-562, RAW264.7
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Informations sur le produit

12490-1-AP cible USP3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP3 Protéine recombinante Ag3085
• Nom complet: ubiquitin specific peptidase 3
• Masse moléculaire calculée: 520 aa, 59 kDa
• Poids moléculaire observé: 53-59 kDa
• Numéro d’acquisition GenBank: BC018113
• Symbole du gène: USP3
• Identification du gène (NCBI): 9960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ; N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors; Authors - Zhishuang Gao
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
human	WB,IHC,IF	Cell Death Dis; USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer; Authors - Sheng Li ; KD Validated
rat	WB	Cell Rep; Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex; Authors - Kellen D Winden
human	WB	BMC Cancer; Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma.; Authors - Ke Shi ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (05-08-2019)

Worked well

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2c12 mouse myoblast

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