Anticorps Polyclonal de lapin anti-USP30

USP30 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 15402-1-AP

Synonymes

Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12

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Galerie de données de validation

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  WB analysis using 15402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15402-1-AP (USP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15402-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of hela using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of HepG2 using 15402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HepG2, cellules hTERT-RPE1, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

15402-1-AP cible USP30 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP30 Protéine recombinante Ag7620
• Nom complet: ubiquitin specific peptidase 30
• Masse moléculaire calculée: 58.5 kDa
• Poids moléculaire observé: 59-67 kDa
• Numéro d’acquisition GenBank: BC004868
• Symbole du gène: USP30
• Identification du gène (NCBI): 84749
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

Publications

Species	Application	Title
human,mouse	WB,IF	Biochem Biophys Res Commun; USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury; Authors - Yang Wu
	IF	Nature; Immune evasion through mitochondrial transfer in the tumour microenvironment; Authors - Hideki Ikeda
human	IHC	Biochim Biophys Acta Mol Basis Dis; FOXF1 promotes ovarian cancer metastasis by facilitating HMGA2-mediated USP30-dependent S100A6 deubiquitination; Authors - Xi Xu
human	WB	J Biochem Mol Toxicol; USP30 Aggravating the Malignant Progression of Breast Cancer and Its Resistance to Tamoxifen by Inhibiting the Ubiquitination of TOMM40; Authors - Xinran Gao

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