Anticorps Polyclonal de lapin anti-USP35

USP35 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 24559-1-AP

Synonymes

EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34

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Galerie de données de validation

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  WB analysis using 24559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24559-1-AP (USP35 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 24559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

24559-1-AP cible USP35 dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP35 Protéine recombinante Ag20002
• Nom complet: ubiquitin specific peptidase 35
• Masse moléculaire calculée: 1018 aa, 113 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC131489
• Symbole du gène: USP35
• Identification du gène (NCBI): 57558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).

Publications

Species	Application	Title
human	WB	Open Life Sci; The deubiquitinating enzyme USP35 regulates the stability of NRF2 protein; Authors - Dian Zhang
human	WB	Commun Biol; Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1; Authors - Shuo Wang ; KD Validated
human	WB,CoIP	Am J Physiol Cell Physiol; USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis; Authors - Weibin Lian
human	WB	Transl Oncol; USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation; Authors - Gen Chen ; KD Validated
human	WB,IHC,CoIP	Cell Death Discov; Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis; Authors - Guowen Lin
human		Oncogenesis; USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer; Authors - Yi Xiao

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