Anticorps Polyclonal de lapin anti-USP35

USP35 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24559-1-PBS

Synonymes

EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34

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Galerie de données de validation

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  WB analysis using 24559-1-AP (same clone as 24559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24559-1-AP (USP35 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 24559-1-AP (same clone as 24559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24559-1-PBS cible USP35 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP35 Protéine recombinante Ag20002
• Nom complet: ubiquitin specific peptidase 35
• Masse moléculaire calculée: 1018 aa, 113 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC131489
• Symbole du gène: USP35
• Identification du gène (NCBI): 57558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).

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