Anticorps Monoclonal anti-USP36

• Phare
• Validé par KD/KO

USP36 Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1B3

N° de cat : 68165-1-PBS

Synonymes

1A1B3, Deubiquitinating enzyme 36, DUB1, EC:2.3.2.-, KIAA1453

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Galerie de données de validation

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  WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68165-1-Ig (same clone as 68165-1-PBS)

  WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68165-1-Ig (same clone as 68165-1-PBS)

  IP result of anti-USP36 (IP:68165-1-Ig, 4ug; Detection:68165-1-Ig 1:3000) with Jurkat cells lysate 2030 ug. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68165-1-Ig (same clone as 68165-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP36 antibody (68165-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 68165-1-Ig (same clone as 68165-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug USP36 Monoclonal antibody (68165-1-Ig, Clone:1A1B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68165-1-PBS cible USP36 dans les applications de WB, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP36 Protéine recombinante Ag6426
• Nom complet: ubiquitin specific peptidase 36
• Masse moléculaire calculée: 1123 aa, 123 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC038983
• Symbole du gène: USP36
• Identification du gène (NCBI): 57602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)

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