Anticorps Recombinant de lapin anti-USP36

USP36 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241962H7

N° de cat : 84620-3-PBS

Synonymes

241962H7, Deubiquitinating enzyme 36, DUB1, EC:2.3.2.-, KIAA1453

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Galerie de données de validation

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  WB analysis using 84620-3-RR (same clone as 84620-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84620-3-RR (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84620-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01428-1

  Sandwich ELISA standard curve of MP01428-1, Human USP36 Recombinant Matched Antibody Pair - PBS only. 84620-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6411. 84620-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01428-1

  The mean USP36 concentration was determined to be 4,390.7 pg/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 16,332.6 pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 3,553.0 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP01428-1

  Cytometric bead array standard curve of MP01428-1, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-1-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84620-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84620-3-RR against Human USP36 were performed. The affinity constant is 3.71 nM.

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Informations sur le produit

84620-3-PBS cible USP36 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: USP36 Protéine recombinante Ag6411
• Nom complet: ubiquitin specific peptidase 36
• Masse moléculaire calculée: 1123 aa, 123 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC038983
• Symbole du gène: USP36
• Identification du gène (NCBI): 57602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.

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