Anticorps Polyclonal de lapin anti-USP38

• Phare
• Validé par KD/KO

USP38 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 17767-1-AP

Synonymes

Deubiquitinating enzyme 38, EC:3.4.19.12, HP43.8KD, KIAA1891, Ubiquitin carboxyl-terminal hydrolase 38

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Galerie de données de validation

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  WB analysis of HEK-293T using 17767-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17767-1-AP (USP38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17767-1-AP

  IP result of anti-USP38 (IP:17767-1-AP, 4ug; Detection:17767-1-AP 1:500) with K-562 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T,
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

17767-1-AP cible USP38 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP38 Protéine recombinante Ag12172
• Nom complet: ubiquitin specific peptidase 38
• Masse moléculaire calculée: 1042 aa, 117 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC068975
• Symbole du gène: USP38
• Identification du gène (NCBI): 84640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin specific peptidase 38 (USP38) is a negative regulator of type I IFN signaling. USP38 can strongly induced the degradation of the active form of TBK1 after viral infection. USP38 may play a critical role in the maintenance of the balance between innate immune responses and tolerance through dynamic ubiquitin editing of TBK1 in response to viral infection and perhaps other infectious diseases or cancer. (PMID:27692986)

Publications

Species	Application	Title
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen ; KO Validated
human	WB,IHC,CoIP	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan ; KD Validated
human	WB,IP,IHC	Am J Cancer Res; Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase.; Authors - Zhi Zheng ; KD Validated
human	WB,IF,CoIP	J Biol Chem; ADAR promotes USP38 auto-deubiquitylation and stabilization in an RNA editing-independent manner in Esophageal Squamous Cell Carcinoma; Authors - Qingyong Hu
human	WB	Int J Biochem Cell Biol; The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.; Authors - Zhijun Xu
rat,mouse	WB,IP,IF	Mol Med; USP38 exacerbates pressure overload-induced left ventricular electrical remodeling; Authors - Yucheng Pan

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