Anticorps Polyclonal de lapin anti-USP39

• Phare
• Validé par KD/KO

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 23865-1-AP

Synonymes

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

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Galerie de données de validation

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  WB analysis of K-562 using 23865-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23865-1-AP

  WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells.

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  WB analysis of HepG2 using 23865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 23865-1-AP

  IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IHC staining of human colon cancer using 23865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 23865-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 23865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 23865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 23865-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A431, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 4 publications below

Informations sur le produit

23865-1-AP cible USP39 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP39 Protéine recombinante Ag20894
• Nom complet: ubiquitin specific peptidase 39
• Masse moléculaire calculée: 565 aa, 65 kDa
• Poids moléculaire observé: 60~65 kDa
• Numéro d’acquisition GenBank: BC067273
• Symbole du gène: USP39
• Identification du gène (NCBI): 10713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2

Publications

Species	Application	Title
human	RIP	Immunity; Lactate modulates RNA splicing to promote CTLA-4 expression in tumor-infiltrating regulatory T cells; Authors - Rui Ding
human	WB	Sci Adv; Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection; Authors - Yiyao Huang
mouse,human	RIP,IHC	Mol Oncol; Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation.; Authors - Yue Xiao ; KD Validated
human	WB	Biomolecules; USP39-Mediated Non-Proteolytic Control of ETS2 Suppresses Nuclear Localization and Activity; Authors - Yunsik Choi
human	WB,IF	Am J Cancer Res; USP39 facilitates breast cancer cell proliferation through stabilization of FOXM1; Authors - Zhenwang Zhang ; KD Validated
human	WB,IHC	Oncotarget; Overexpression of USP39 predicts poor prognosis and promotes tumorigenesis of prostate cancer via promoting EGFR mRNA maturation and transcription elongation.; Authors - Yi Huang ; KD Validated

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