Anticorps Polyclonal de lapin anti-USP39

• Phare
• Validé par KD/KO

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23865-1-PBS

Synonymes

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

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Galerie de données de validation

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  WB analysis of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23865-1-AP (same clone as 23865-1-PBS)

  WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23865-1-AP (same clone as 23865-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 23865-1-AP (same clone as 23865-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

  IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23865-1-PBS cible USP39 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP39 Protéine recombinante Ag20894
• Nom complet: ubiquitin specific peptidase 39
• Masse moléculaire calculée: 565 aa, 65 kDa
• Poids moléculaire observé: 60~65 kDa
• Numéro d’acquisition GenBank: BC067273
• Symbole du gène: USP39
• Identification du gène (NCBI): 10713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2

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