Anticorps Monoclonal anti-USP4

USP4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1G11G8

N° de cat : 66822-1-Ig

Synonymes

Deubiquitinating enzyme 4, Ubiquitin thiolesterase 4, UNP, UnpEL, Unph, USP4

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Galerie de données de validation

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  WB analysis using 66822-1-Ig

  HEK-293 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 66822-1-Ig

  NCI-H1299 cells and RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66822-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66822-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66822-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 66822-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of THP-1 using 66822-1-Ig

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human USP4 (66822-1-Ig, Clone:1G11G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules NCI-H1299, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain, tissu de cancer du foie humain
• Résultats positifs en cytométrie: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:300
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66822-1-Ig cible USP4 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP4 Protéine recombinante Ag28297
• Nom complet: ubiquitin specific peptidase 4 (proto-oncogene)
• Masse moléculaire calculée: 963 aa, 109 kDa
• Poids moléculaire observé: 109 kDa
• Numéro d’acquisition GenBank: BC125131
• Symbole du gène: USP4
• Identification du gène (NCBI): 7375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP4, also named as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

Publications

Species	Application	Title
human	IHC	Cell Death Dis; USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1; Authors - Hongdian Zhang
mouse	WB	bioRxiv; The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice; Authors - Tegan S Horan
human	WB,IF,IP	PLoS One; USP4 promotes the proliferation and glucose metabolism of gastric cancer cells by upregulating PKM2; Authors - Yuanyuan Chen