Anticorps Polyclonal de lapin anti-USP48

USP48 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 12076-1-AP

Synonymes

Deubiquitinating enzyme 48, EC:3.4.19.12, RAP1GA1, Ubiquitin carboxyl-terminal hydrolase 48, Ubiquitin thiolesterase 48

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Galerie de données de validation

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  WB analysis of PC-3 using 12076-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12076-1-AP (USP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12076-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12076-1-AP (USP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12076-1-AP

  IP result of anti-USP48 (IP:12076-1-AP, 4ug; Detection:12076-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 12076-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12076-1-AP (USP48 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12076-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12076-1-AP (USP48 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12076-1-AP cible USP48 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: USP48 Protéine recombinante Ag2749
• Nom complet: ubiquitin specific peptidase 48
• Masse moléculaire calculée: 1035 aa, 119 kDa
• Poids moléculaire observé: 119 kDa
• Numéro d’acquisition GenBank: BC013567
• Symbole du gène: USP48
• Identification du gène (NCBI): 84196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.

Publications

Species	Application	Title
human	WB,IP,IHC,IF	Acta Pharm Sin B; Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis; Authors - Can Cheng
	WB	Cancer Res; YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.; Authors - Shuai Ye
human	IHC	Rheumatology (Oxford); Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.; Authors - Christine Galant
mouse	IP	Eur J Pain; USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice; Authors - Jiacheng Yu
mouse	WB	FASEB J; Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination; Authors - Ping Wang
human	CoIP	Sci Rep; RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31; Authors - Wei Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachel (Verified Customer) (09-10-2020)

Antibody detected band of the expected height by western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

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