Anticorps Polyclonal de lapin anti-USP48
USP48 Polyclonal Antibody for WB, IHC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 12076-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules PC-3, cellules HeLa |
Résultats positifs en IP | cellules HeLa |
Résultats positifs en IHC | tissu de cancer du pancréas humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Informations sur le produit
12076-1-AP cible USP48 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | USP48 Protéine recombinante Ag2749 |
Nom complet | ubiquitin specific peptidase 48 |
Masse moléculaire calculée | 1035 aa, 119 kDa |
Poids moléculaire observé | 119 kDa |
Numéro d’acquisition GenBank | BC013567 |
Symbole du gène | USP48 |
Identification du gène (NCBI) | 84196 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.
Protocole
Product Specific Protocols | |
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WB protocol for USP48 antibody 12076-1-AP | Download protocol |
IHC protocol for USP48 antibody 12076-1-AP | Download protocol |
IP protocol for USP48 antibody 12076-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Cancer Res YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis. | ||
Rheumatology (Oxford) Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β. | ||
Eur J Pain USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice | ||
FASEB J Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination | ||
Sci Rep RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31 |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rachel (Verified Customer) (09-10-2020) | Antibody detected band of the expected height by western blot.
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